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Human BTD (Biotinidase) Microsample ELISA Kit

CAT: 0013-GTR18872536-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872536-01Size:48 Tests
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Description
Human BTD (Biotinidase) Microsample ELISA Kit
Product Name Alternative
Biotinase
UniProt
P43251
Reactivity
Human
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.058 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BTD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BTD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BTD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BTD in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P43251

Biotinidase

BTD_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P43251
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BTD
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Biotinase
EC number
3.5.1.12
Processing
Precursor
Secondary accessions
A6NHF2, B2R865, B4DFX1, B4DLJ9, B7Z7C9, F8W1Q3, Q96EM9
Protein keywords

Coding sequence diversity

Alternative splicing

Technical term

Direct protein sequencingProteomics identificationReference proteome

Disease

Disease variant

PTM

Glycoprotein

Molecular function

Hydrolase

Cellular component

Secreted

Domain

Signal

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