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Human PEX2 (Peroxisomal Biogenesis Factor 2) Microsample ELISA Kit

CAT: 0013-GTR18872445-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872445-01Size:48 Tests
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Description
Human PEX2 (Peroxisomal Biogenesis Factor 2) Microsample ELISA Kit
Product Name Alternative
PXMP3; PAF1; PMP35; PMP3; RNF72; Peroxin 2; Peroxisome Assembly Factor 1; Peroxisomal Membrane Protein 35kDa; Peroxisomal Membrane Protein 3
UniProt
P28328
Reactivity
Human
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.061 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEX2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PEX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEX2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P28328

Peroxisome biogenesis factor 2

PEX2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28328
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PEX2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.2.27, 2.3.2.36
Processing
—
Secondary accessions
Q567S6, Q9BW41
Protein keywords

PTM

AcetylationDisulfide bond

Disease

Disease variantPeroxisome biogenesis disorderZellweger syndrome

Cellular component

MembranePeroxisome

Ligand

Metal-bindingZinc

Biological process

Peroxisome biogenesisProtein transportTransportUbl conjugation pathway

Technical term

Proteomics identificationReference proteome

Molecular function

Transferase

Domain

TransmembraneTransmembrane helixZinc-finger

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