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Rat CBG (Corticosteroid Binding Globulin) Microsample ELISA Kit

CAT: 0013-GTR18872326-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872326-01Size:48 Tests
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Description
Rat CBG (Corticosteroid Binding Globulin) Microsample ELISA Kit
Product Name Alternative
SERPINA6; Transcortin; Serpin A6; Serpin Peptidase Inhibitor, Clade A, Member 6 (Alpha-1 Antiproteinase; Antitrypsin)
UniProt
P31211
Reactivity
Rat
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
6.7 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CBG in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 ng/mL

UniProtKB · P31211

Corticosteroid-binding globulin

CBG_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P31211
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Serpina6
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
CBG
EC number
—
Processing
Precursor
Secondary accessions
Q5M822
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

PTM

Glycoprotein

Ligand

Lipid-bindingSteroid-binding

Cellular component

Secreted

Domain

Signal

Biological process

Transport

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