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Human PIM2 (Pim-2 Oncogene) Microsample ELISA Kit

CAT: 0013-GTR18872186-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872186-01Size:48 Tests
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Description
Human PIM2 (Pim-2 Oncogene) Microsample ELISA Kit
Product Name Alternative
Serine/threonine-protein kinase pim-2
UniProt
Q9P1W9
Reactivity
Human
Field of Research
Cancer Biology; Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.236 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIM2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.63-40 ng/mL

UniProtKB · Q9P1W9

Serine/threonine-protein kinase pim-2

PIM2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9P1W9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PIM2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
A8K4G6, Q99739
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

ApoptosisCell cycle

Ligand

ATP-bindingNucleotide-binding

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

PTM

Phosphoprotein

Disease

Proto-oncogene

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