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Mouse EREG (Epiregulin) Microsample ELISA Kit

CAT: 0013-GTR18872071-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872071-01Size:48 Tests
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Description
Mouse EREG (Epiregulin) Microsample ELISA Kit
Product Name Alternative
EPR; ER; Proepiregulin
UniProt
Q61521
Reactivity
Mouse
Field of Research
Cardiovascular Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
6 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EREG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EREG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EREG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EREG in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000 pg/mL

UniProtKB · Q61521

Proepiregulin

EREG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61521
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ereg
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q7M0A8
Protein keywords

Biological process

AngiogenesisDifferentiation

Cellular component

Cell membraneMembraneSecreted

Molecular function

Developmental proteinGrowth factorMitogen

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignalTransmembraneTransmembrane helix

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