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Mouse SST (Somatostatin) Microsample ELISA Kit

CAT: 0013-GTR18871790-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871790-01Size:48 Tests
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Description
Mouse SST (Somatostatin) Microsample ELISA Kit
Product Name Alternative
GHIH; SRIF; SMST; GHRIH; GHRIF; Growth Hormone Releasing Inhibiting Hormone; Somatotropin Release-Inhibiting Factor; Somatostatin-14; Somatostatin-28
UniProt
P60041
Reactivity
Mouse
Field of Research
Stem Cell & Developmental Biology; Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Sensitivity
3.1 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SST in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
7.82-500 pg/mL

UniProtKB · P60041

Somatostatin

SMS_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P60041
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Sst
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
P01167
Protein keywords

PTM

AmidationCleavage on pair of basic residuesDisulfide bond

Molecular function

Hormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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