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Mouse FLG (Filaggrin) Microsample ELISA Kit

CAT: 0013-GTR18871587-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871587-01Size:48 Tests
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Description
Mouse FLG (Filaggrin) Microsample ELISA Kit
Product Name Alternative
Profilaggrin
UniProt
P11088
Reactivity
Mouse
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.108 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse FLG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse FLG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse FLG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse FLG in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P11088

Filaggrin

FILA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P11088
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Flg
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Fragment
Secondary accessions
—
Protein keywords

Molecular function

Developmental protein

PTM

Phosphoprotein

Technical term

Reference proteome

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