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E. coli ligA protein

CAT: 0013-GTR18880877-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR18880877-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This E. coli ligA protein spans the amino acid sequence from region 1-671aa. Purity: Greater than 90% as determined by SDS-PAGE.
Product Name Alternative
LigA dnaL lig lop pdeC b2411 JW2403
UniProt
P15042
Expression Region
1-671aa
Tag
N-terminal 6xHis-tagged
Source
E.coli
Origin
Escherichia coli (strain K12)
Field of Research
Infectious Disease & Virology
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Molecular Weight
77.6 kDa
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes
For research use only.
Applications Notes
Full length of His-tag and expression region is 1-671aa
Preservative
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
AA Sequence
MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRELETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEEDRPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVLSERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMDVDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAEESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVELGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS

UniProtKB · P15042

DNA ligase

DNLJ_ECOLI · Escherichia coli (strain K12)

View on UniProt ↗
Primary accession
P15042
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ligA
Protein existence
1: Evidence at protein level
Organism
Escherichia coli (strain K12)
Taxonomy ID
83333
Alternative names
—
EC number
6.5.1.2
Processing
—
Secondary accessions
P78197, P78198
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Biological process

DNA damageDNA repairDNA replication

Molecular function

Ligase

Ligand

MagnesiumMetal-bindingNADZinc

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