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Klenow Fragment

CAT: 0013-GTR18878336Size: 5 x 300 unitsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878336Size:5 x 300 units
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Klenow Fragment is the large fragment of DNA Polymerase I that retains its 5'→3' polymerase, 3'→5' exonuclease and strand displace-ment activities. The enzyme lacks the 5'→3' exonuclease activity of intact DNA polymerase I. Klenow retains the polymerization fidelity of the holoenzyme without degrading 5' termini.
Product Name Alternative
Large Fragment of DNA Polymerase I, recombinant, E. coli
Concentration
5 units/μl
Purity
The enzyme is greater than 98% pure as indicated by SDS-
Form
Liquid (Supplied in 100 mM KPO 4pH 6.5, 1 mM DTT and 50% [v/v] glycerol)
Storage Conditions
Store at -20 °C
Notes
For research use only.
Applications Notes
Fill-in of 5' overhangs to form blunt ends. Removal of 3' overhangs to form blunt ends.

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