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Human IL-1β ELISA Kit

CAT: 0013-GTR18878547-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18878547-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate Human IL-1β.
Product Name Alternative
Interleukin 1β; IL1B; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta
Reactivity
Human
Sample Type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Sensitivity
4 pg/mL
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Notes
For research use only.
Applications Notes
Human IL-1β ELISA Kit employs a two-site sandwich ELISA to quantitate IL-1β in samples. An antibody specific for IL-1β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1β is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1β bound in the initial step. The color development is stopped and the intensity of the color is measured.
Tested Applications
ELISA
Dynamic Range
7.8 pg/ml-500 pg/ml

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