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Human TRAF2 (TNF Receptor-Associated Factor 2) Microsample ELISA Kit

CAT: 0013-GTR18871755-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871755-01Size:48 Tests
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Description
Human TRAF2 (TNF Receptor-Associated Factor 2) Microsample ELISA Kit
Product Name Alternative
TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3
UniProt
Q12933
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
25 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TRAF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TRAF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TRAF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TRAF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000 pg/mL

UniProtKB · Q12933

TNF receptor-associated factor 2

TRAF2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q12933
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
TRAF2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.3.2.27
Processing
—
Secondary accessions
A8K107, B4DPJ7, Q7Z337, Q96NT2
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisUbl conjugation pathway

Domain

Coiled coilRepeatZinc-finger

Cellular component

Cytoplasm

Ligand

Lipid-bindingMetal-bindingZinc

Molecular function

Transferase

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