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Human IL28B (Interleukin 28B) Microsample ELISA Kit

CAT: 0013-GTR18873150-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873150-01Size:48 Tests
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Description
Human IL28B (Interleukin 28B) Microsample ELISA Kit
Product Name Alternative
IL-28B; IFNL3; Interferon, Lambda 3; Cytokine Zcyto22
UniProt
Q8IZI9
Reactivity
Human
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
3 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL28B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL28B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL28B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL28B in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
7.82-500 pg/mL

UniProtKB · Q8IZI9

Interferon lambda-3

IFNL3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8IZI9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNL3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-lambda-3
EC number
—
Processing
Precursor
Secondary accessions
A2BDE1, Q6VN56, Q7Z4J3, Q8IWL6
Protein keywords

Technical term

3D-structureReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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