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Rat PCNA (Proliferating Cell Nuclear Antigen) Microsample ELISA Kit

CAT: 0013-GTR18872213-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872213-01Size:48 Tests
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Description
Rat PCNA (Proliferating Cell Nuclear Antigen) Microsample ELISA Kit
Product Name Alternative
Cyclin
UniProt
P04961
Reactivity
Rat
Field of Research
Immunology & Inflammation; Epigenetics & Chromatin
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.054 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PCNA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PCNA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PCNA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PCNA in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P04961

DNA sliding clamp PCNA

PCNA_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P04961
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pcna
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

PTM

AcetylationDisulfide bondIsopeptide bondMethylationPhosphoproteinUbl conjugation

Biological process

DNA damageDNA repairDNA replication

Molecular function

DNA-binding

Cellular component

Nucleus

Technical term

Reference proteome

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