Biotinylated Human Siglec-15 / CD33L3 Protein, Fc,Avitag™

Biotinylated Human Siglec-15 / CD33L3 Protein, Fc,Avitag™

• Background: Siglec-15 is a DAP12-associated immunoreceptor, which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family. Siglecs are cell surface proteins that bind sialic acid. They are found primarily on the surface of immune cells and are a subset of the I-type lectins. Siglec-15 consisting of immunoglobulin (Ig)-like domains, transmembrane domain and a short cytoplasmic tail. Siglec-15 is that recognizes sialylated glycans and regulates osteoclast differentiation. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.
• Description: Biotinylated Human Siglec-15, Fc,Avitag (SG5-H82F5) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Thr 263 (Accession # Q6ZMC9-1).
• Specifications: This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 54.8 kDa. The protein migrates as 58 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Phe 20 - Thr 263
• Host: HEK 293
• Target: Siglec-15
• Conjugation: Biotin-labeled
• Tag: C-Fc & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Mouse Human chimeric MAb (5G12, Human IgG1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Siglec-15, Fc,Avitag (Cat. No. SG5-H82F5) with a linear range of 0.4-3 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 54.8 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C