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OGT Antibody

CAT: 0013-GTR17697182Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17697182Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to OGT
Product Name Alternative
Anti OGT antibody, anti O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase) antibody, anti FLJ23071 antibody, anti HRNT1 antibody, anti MGC22921 antibody, anti O-GLCNAC antibody, anti O-GlcNAc transferase p110 subunit O-linked GlcNAc transferase antibody, anti uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase antibody
Reactivity
Human, Rat
Target
OGT
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
YEHPKDLKLSDGR
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:64000, WB: 0.05-0.2 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
117, 116
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 110kDa band observed in Pancreas lysates from Rat and Mouse (calculated MW of 117kDa according to NP_858058.1. Recommended concentration: 0.05-0.2 μg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse
Tested Applications
ELISA, FC, IF, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
8473

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