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Mouse Cyclic AMP Response Element Binding Protein (CREB) ELISA Kit

CAT: 0013-GTR18864333-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18864333-01Size:48 Tests
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Description
Mouse Cyclic AMP Response Element Binding Protein (CREB) ELISA Kit
Product Name Alternative
CREB; active transcription factor CREB; cAMP responsive element binding protein 1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic AMP-responsive element-binding protein 1; MGC9284; transactivator protein
UniProt
Q01147
Reactivity
Mouse
Field of Research
Stem Cell & Developmental Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.049 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CREB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CREB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CREB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CREB in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.16-10 ng/mL

UniProtKB · Q01147

Cyclic AMP-responsive element-binding protein 1

CREB1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q01147
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Creb1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
CREB-1, cAMP-responsive element-binding protein 1
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

ActivatorDNA-binding

Coding sequence diversity

Alternative splicing

Biological process

Biological rhythmsDifferentiationTranscriptionTranscription regulation

PTM

Isopeptide bondPhosphoproteinUbl conjugation

Cellular component

Nucleus

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