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Mouse Alpha-2-Macroglobulin (a2M) ELISA Kit

CAT: 0013-GTR18864239-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18864239-01Size:48 Tests
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Description
Mouse Alpha-2-Macroglobulin (a2M) ELISA Kit
Product Name Alternative
α2MG; α2Mg; CPAMD5; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
UniProt
Q6GQT1
Reactivity
Mouse
Field of Research
Cancer Biology, Hematology, Infectious Disease & Virology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, urine and other biological fluids
Sensitivity
2.1 μg/mL
Notes
For research use only.
Applications Notes
Standard: 500 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse a2M. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse a2M. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse a2M, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse a2M in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.82-500 μg/mL

UniProtKB · Q6GQT1

Alpha-2-macroglobulin-P

A2MG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q6GQT1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
A2m
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
E9QMQ7, Q811S0
Protein keywords

Domain

Bait regionSignal

PTM

Disulfide bondGlycoproteinThioester bond

Biological process

Pregnancy

Molecular function

Protease inhibitorSerine protease inhibitor

Technical term

Reference proteome

Cellular component

Secreted

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