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Mouse Protectin (CD59) ELISA Kit

CAT: 0013-GTR18863859-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863859-01Size:48 Tests
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Description
Mouse Protectin (CD59) ELISA Kit
Product Name Alternative
MIC11; MIN1; MIN2; MIN3; MSK21; MAC-IP; MACIF; MIRL; HRF20; Complement Regulatory Protein; 20 kDa homologous restriction factor; Membrane attack complex inhibition factor
UniProt
O55186
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, urine and other biological fluids.
Sensitivity
0.11 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD59. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD59. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD59, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD59 in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.32-20 ng/mL

UniProtKB · O55186

CD59A glycoprotein

CD59A_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O55186
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cd59a
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q542R7
Protein keywords

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Technical term

Reference proteome

Domain

Signal

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