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Rat Complement Component 4 (C4) ELISA Kit

CAT: 0013-GTR18863452-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863452-01Size:48 Tests
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Description
Rat Complement Component 4 (C4) ELISA Kit
Product Name Alternative
C4BP-a; Complement Component 4 Binding Protein, Alpha
UniProt
P08649
Reactivity
Rat
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids
Sensitivity
2.25 μg/mL
Notes
For research use only.
Applications Notes
Standard: 500 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat C4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat C4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat C4 in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
7.82-500 μg/mL

UniProtKB · P08649

Complement C4

CO4_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P08649
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C4
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q62895, Q8R403
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoproteinSulfationThioester bond

Biological process

Complement pathwayImmunityInflammatory responseInnate immunity

Technical term

Direct protein sequencingReference proteome

Cellular component

Secreted

Domain

Signal

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