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Mouse Thyroxine Binding Globulin (TBG) ELISA Kit

CAT: 0013-GTR18863420-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18863420-01Size:48 Tests
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Description
Mouse Thyroxine Binding Globulin (TBG) ELISA Kit
Product Name Alternative
SERPINA7; Serpin Peptidase Inhibitor Clade A Member 7; T4-binding globulin
UniProt
P61939
Reactivity
Mouse
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
0.051 μg/mL
Notes
For research use only.
Applications Notes
Standard: 10 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TBG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TBG in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.16-10 μg/mL

UniProtKB · P61939

Thyroxine-binding globulin

THBG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P61939
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Serpina7
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A2RSG7
Protein keywords

PTM

Glycoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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