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Simian CFB (Complement Factor B) ELISA Kit

CAT: 0013-GTR18860554-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18860554-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CFB in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CF-B; BF; BFD; CFAB; GBG; H2-Bf; PBF2; B-Factor; Properdin; Properdin factor B; C3/C5 convertase; Glycine-rich beta glycoprotein
Reactivity
Primate
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.12 μg/mL
Notes
For research use only.
Applications Notes
Standard: 20 μg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian CFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian CFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian CFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian CFB in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.32-20 μg/mL

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