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Mouse Complement Component 4a (C4a) ELISA Kit

CAT: 0013-GTR18859152-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18859152-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Mouse Complement Component 4a (C4a) ELISA Kit
Product Name Alternative
C4AD, C4S, CO4, CPAMD2, RG
UniProt
P01029
Reactivity
Mouse
Target
C4a
Field of Research
Neuroscience; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
46.88 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C4a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C4a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C4a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C4a. You can calculate the concentration of Mouse C4a in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
78.13-5000pg/mL

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