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Human Complement Component 7 (C7) ELISA Kit

CAT: 0013-GTR18858629-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18858629-01Size:48 Tests
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Description
Human Complement Component 7 (C7) ELISA Kit
Product Name Alternative
C7
UniProt
P10643
Reactivity
Human
Target
C7
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
7.5 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C7. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C7. You can calculate the concentration of Human C7 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
12.5-800pg/mL

UniProtKB · P10643

Complement component C7

CO7_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P10643
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C7
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8K2T4, Q6P3T5, Q92489
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Complement alternate pathwayComplement pathwayCytolysisImmunityInnate immunity

Disease

Disease variant

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainRepeatSignalSushi

Cellular component

MembraneMembrane attack complexSecretedTarget cell membraneTarget membrane

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