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Human Complement Fragment 3c (C3c) ELISA Kit

CAT: 0013-GTR18858623-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18858623-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This kit is designed for detection of free C3c. It exhibits range of 0.32-20ng/mL, suitable sample types including serum, plasma, tissue homogenate and other biological samples.
Product Name Alternative
C3c
UniProt
P01024
Reactivity
Human
Target
C3c
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, tissue homogenate and other biological samples
Sensitivity
0.19 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C3c. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C3c and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C3c, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human C3c. You can calculate the concentration of Human C3c in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20ng/mL

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