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Human Immunoglobulin E (IgE) ELISA Kit

CAT: 0013-GTR18856939-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18856939-01Size:48 Tests
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Description
Human Immunoglobulin E (IgE) ELISA Kit
Product Name Alternative
IgE
UniProt
P01854
Reactivity
Human
Target
IgE
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
1.88 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IgE. You can calculate the concentration of Human IgE in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
3.13-200ng/mL

UniProtKB · P01854

Immunoglobulin heavy constant epsilon

IGHE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01854
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IGHE
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A0A286YES5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Adaptive immunityImmunityInflammatory response

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneImmunoglobulinMembraneSecreted

PTM

Disulfide bondGlycoprotein

Domain

Immunoglobulin domainRepeatTransmembraneTransmembrane helix

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