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Alpha-B Crystallin ELISA Kit

CAT: 0013-GTR18855587Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18855587Size:96 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Colorimetric detection of alpha B Crystallin
Product Name Alternative
AACRYA, CRYA2, CRYAB, CTPP2, HSPB5, NY Ren 27 antigen, alpha crystallin B
Reactivity
Human
Target
Alpha-B Crystallin ELISA Kit
Field of Research
Cancer Biology, Cell Biology
Assay Type
Sandwich
Sample Type
Cell Lysates, Tissue, Serum
Sensitivity
0.009 ng/ml
Storage Conditions
4°C and -20°C
Notes
For research use only.
Applications Notes
1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 μL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 μL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 μL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 μL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 μL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Dynamic Range
0.352 - 22.5 ng/ml

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