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Mouse Beta-Endorphin (β-EP) ELISA Kit

CAT: 0013-GTR18854681-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854681-01Size:48 Tests
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Description
Mouse Beta-Endorphin (β-EP) ELISA Kit
Product Name Alternative
B-EP
UniProt
P01193
Reactivity
Mouse
Target
β-EP
Field of Research
Metabolism Research
Assay Type
Competitive
Assay Performance Time
1.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
9.38 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse β-EP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Mouse β-EP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse β-EP in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000pg/mL

UniProtKB · P01193

Pro-opiomelanocortin

COLI_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01193
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Pomc
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
POMC
EC number
—
Processing
Precursor
Secondary accessions
P01200, Q544U4
Protein keywords

PTM

AcetylationAmidationCleavage on pair of basic residuesGlycoproteinPhosphoprotein

Molecular function

EndorphinHormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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