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Human Pepsin (PP) ELISA Kit

CAT: 0013-GTR18854265-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854265-01Size:48 Tests
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Description
Human Pepsin (PP) ELISA Kit
Product Name Alternative
Pepsin
UniProt
P01298
Reactivity
Human
Target
PP
Field of Research
Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
25.2 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PP. You can calculate the concentration of Human PP in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
62.5-4000pg/mL

UniProtKB · P01298

Pancreatic polypeptide prohormone

PAHO_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01298
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PPY
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PH
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingPharmaceuticalProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

AmidationCleavage on pair of basic residues

Molecular function

Hormone

Cellular component

Secreted

Domain

Signal

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