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Mouse Interleukin 3 (IL-3) Microsample Fast ELISA Kit

CAT: 0013-GTR18853793-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853793-01Size:48 Tests
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Description
Mouse Interleukin 3 (IL-3) Microsample Fast ELISA Kit
Product Name Alternative
IL3, MCGF, MCSF, MULTI-CSF, P-Cell Stimulating Factor
UniProt
P01586
Reactivity
Mouse
Target
IL-3
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples; Sample volume:25μL
Sensitivity
9.38 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-3. You can calculate the concentration of Mouse IL-3 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000pg/mL

UniProtKB · P01586

Interleukin-3

IL3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01586
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-3
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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