Products for Research Use Only

Human Interleukin 5 (IL-5) Microsample Fast ELISA Kit

CAT: 0013-GTR18853792-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853792-01Size:48 Tests
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Description
Human Interleukin 5 (IL-5) Microsample Fast ELISA Kit
Product Name Alternative
IL5, EDF, TRF, E-CSF, ESGF
UniProt
P05113
Reactivity
Human
Target
IL-5
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples; Sample volume:25μL
Sensitivity
9.38 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-5. You can calculate the concentration of Human IL-5 in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
15.63-1000pg/mL

UniProtKB · P05113

Interleukin-5

IL5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05113
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-5
EC number
—
Processing
Precursor
Secondary accessions
Q13840
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal