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General Cortisol (Cor) ELISA Kit

CAT: 0013-GTR18853675-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853675-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
General Cortisol (Cor) ELISA Kit
Product Name Alternative
Hydrocortisone
Reactivity
All
Target
Cor
Field of Research
Metabolism Research
Assay Type
Competitive
Assay Performance Time
1.5H
Sample Type
Serum, plasma, urine and other biological fluids
Sensitivity
0.19 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Cor. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Cor. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cor in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.31-20ng/mL

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