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Mouse Dopamine Transporter (DAT) ELISA Kit

CAT: 0013-GTR18858384-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18858384-01Size:48 Tests
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Description
Mouse Dopamine Transporter (DAT) ELISA Kit
Product Name Alternative
Dopamine active transporter, SLC6A3, solute carrier family 6 (neurotransmitter transporter), member 3, DAT1, PKDYS, solute carrier family 6 member 3, Sodium-dependent dopamine transporter, DA transporter
UniProt
Q61327
Reactivity
Mouse
Target
DAT
Field of Research
Neuroscience; Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
4.69 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DAT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DAT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse DAT. You can calculate the concentration of Mouse DAT in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
7.82-500pg/mL

UniProtKB · Q61327

Sodium-dependent dopamine transporter

SC6A3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q61327
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Slc6a3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
DA transporter, DAT
EC number
—
Processing
—
Secondary accessions
Q60719, Q9R1I2
Protein keywords

Cellular component

Cell membraneCell projectionMembrane

PTM

Disulfide bondGlycoprotein

Ligand

Metal-bindingSodium

Biological process

Neurotransmitter transportSymportTransport

Technical term

Reference proteome

Domain

TransmembraneTransmembrane helix

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