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Mouse Leptin (LEP) ELISA Kit

CAT: 0013-GTR18854853-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854853-01Size:48 Tests
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Description
Mouse Leptin (LEP) ELISA Kit
Product Name Alternative
LEPD, OB, OBS, Obesity Homolog
UniProt
P41160
Reactivity
Mouse
Target
LEP
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
0.19 ng/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
0.31-20ng/mL

UniProtKB · P41160

Leptin

LEP_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P41160
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lep
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Disease

Diabetes mellitusObesity

PTM

Disulfide bond

Cellular component

Secreted

Domain

Signal

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