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Human Interferon Beta (IFN-β) ELISA Kit

CAT: 0013-GTR18854483-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18854483-01Size:48 Tests
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Description
Human Interferon Beta (IFN-β) ELISA Kit
Product Name Alternative
IFNB1, IFB, IFF, IFNB
UniProt
P01574
Reactivity
Human
Target
IFNb
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Macrophage culture supernatant, bone marrow mesenchymal stem cell supernatant
Sensitivity
13.7 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IFN-β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IFN-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IFN-β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IFN-β. You can calculate the concentration of Human IFN-β in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000pg/mL

UniProtKB · P01574

Interferon beta

IFNB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01574
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IFNB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IFN-beta
EC number
—
Processing
Precursor
Secondary accessions
Q5VWC9
Protein keywords

Technical term

3D-structurePharmaceuticalProteomics identificationReference proteome

Biological process

Antiviral defense

Molecular function

Cytokine

PTM

Disulfide bondGlycoproteinPhosphoprotein

Cellular component

Secreted

Domain

Signal

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