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Rat Osteonectin (ON) ELISA Kit

CAT: 0013-GTR18858273-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18858273-01Size:48 Tests
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Description
Rat Osteonectin (ON) ELISA Kit
Product Name Alternative
SPARC
UniProt
P09486
Reactivity
Rat
Target
ON
Field of Research
Gastroenterology & Hepatology, Metabolism Research, Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5H
Sample Type
Serum, plasma, Tissue homogenate and Other biological samples
Sensitivity
18.75 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ON. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ON and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ON, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ON. You can calculate the concentration of Rat ON in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
31.25-2000pg/mL

UniProtKB · P09486

SPARC

SPRC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P09486
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SPARC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D3DQH9, Q6IBK4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Basement membraneExtracellular matrixSecreted

Ligand

CalciumCopperMetal-binding

Disease

Disease variantOsteogenesis imperfecta

PTM

Disulfide bondGlycoprotein

Domain

Signal

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