Products for Research Use Only

EYA4 Antibody

CAT: 0013-GTR17696981Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17696981Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Goat polyclonal antibody to EYA4
Product Name Alternative
Anti EYA4 antibody, anti eyes absent homolog 4 (Drosophila) antibody, anti HGNC:3522 antibody, anti DFNA10 antibody, anti OTTHUMP00000040267 antibody, anti dJ78N10.1 (eyes absent (Drosophila) homolog 4) antibody, anti deafness, autosomal dominant 10 antibody, anti eyes absent 4 antibody, anti RP11-704J17.4 antibody, anti CMD1J antibody, anti DFNA10 antibody
Reactivity
Human
Target
EYA4
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EDSQDLNEQSVKKTC
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, IHC-P: 3.75 μg/ml
Molecular Weight
69.5; ; 69..4; 67.1; 63.9; 70.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 50kDa band in human and mouse brain lysates and in pituitary mouse cell line NIH 3T3 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated sizes of 69.5kDa, 67.1kDa and 69.4kDa according to NP_004091.2, NP_742102.1 and NP_742103.1 respectively. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Mouse
Tested Applications
ELISA, IHC
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
2070