Recombinant Mouse IGF-1/Human HSA Fusion Protein (His Tag)

• Background:

  IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II (IGF-I and IGF-II), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

• Abbreviation:

  IGF-1/HSA

• Synonyms:

  Insulin-like growth factor I; Somatomedin C; Mechano growth factor

• UniProt:

  P05017/P02768

• Accession Number:

  P05017/P02768

• Expression System:

  Mammalian

• Tag:

  C-His

• Sequence:

  IGF-1 (Gly49-Ala118) /HSA (Met1-Asp586)

• Field of Research:

  Metabolism; Developmental Biology; Signal Transduction

• Endotoxin:

  < 1.0 EU/mg of the protein as determined by the LAL method

• Purity:

  > 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  70 kDa

• Observed Molecular Weight:

  70 kDa

• Formulation:

  Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

• Species:

  Mouse