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Mouse Complement Component 1, Q Subcomponent A (C1qA) ELISA Kit

CAT: 0013-GTR18834426-02Size: 96 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18834426-02Size:96 Tests
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Description
Mouse C1qA ELISA Kit
Product Name Alternative
Complement Component 1, q Subcomponent, Alpha Polypeptide
UniProt
P98086
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma and other biological fluids.
Sensitivity
0.059 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse C1qA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse C1qA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse C1qA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse C1qA in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
0.32-20 ng/mL

UniProtKB · P98086

Complement C1q subcomponent subunit A

C1QA_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P98086
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C1qa
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q9DCM6
Protein keywords

Ligand

CalciumMetal-binding

Domain

CollagenRepeatSignal

Biological process

Complement pathwayImmunityInnate immunity

PTM

Disulfide bondGlycoproteinHydroxylation

Technical term

Reference proteome

Cellular component

Secreted

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