Uncoated Mouse IL-3 ( Interleukin 3 ) ELISA Kit

Uncoated Mouse IL-3 ( Interleukin 3 ) ELISA Kit

• Abbreviation: IL-3
• Synonyms: IL3; MCGF; MCSF; MULTI-CSF; P-Cell Stimulating Factor
• CAS Number: 7732-18-5
• UniProt: P01586
• Cross Reactivity: This kit recognizes IL-3 in samples.No significant cross-reactivity or interference between IL-3 and analogues was observed.
• Target: Mouse
• Type: Sandwich-ELISA
• Applications: ELISA
• Field of Research: Cell Biology; Signal Transduction; Stem Cells
• Detection Method: Colorimetric method; ELISA; Sandwich
• Assay Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of IL-3 . You can calculate the concentration of IL-3 in the samples by comparing the OD of the samples to the standard curve.
• Assay Performance Time: 24 h
• Sample Type: Serum, plasma and other biological fluids
• Detection Range: 1.25-80 pg/mL
• Precision: Both intra-CV and inter-CV are < 10%
• Recovery: 80%-120%
• Shipping Conditions: Ice bag
• Storage Conditions: -20°C,12 months
• Applications Notes: This ELISA kit applies to the in vitro quantitative determination of Mouse IL-3 concentrations in serum, plasma and other biological fluids.