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Basic Calcein AM Cell Viability Kit

CAT: 0013-GTR18821996Size: 25 - 250 TDry Ice: NoHazardous: No
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CAT#:0013-GTR18821996Size:25 - 250 T
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This kit allows researchers to easily and simultaneously differentiate between live and dead cells within a single sample. Samples can be analyzed using a flow cytometer, fluorescence plate reader, or fluorescent microscope.
Field of Research
Neuroscience
Sample Type
Cell culture
Storage Conditions
Calcein AM at ≤-20°C, other components at 2-8°C
Notes
For research use only.
Applications Notes
1. Prepare samples and controls. 2. Dilute 10X Cellular Assay Buffer 1:10 with diH2O. 3. Reconstitute Calcein AM with 50 μL DMSO. 4. Dilute 2 mM Calcein AM stock solution 1:5 by adding 200 μL diH2O or PBS, forming a 400 μM stock solution. 5. Stain with Calcein AM at a concentration between 1-10 μM. The ideal staining concentration can vary based on cell line, application, etc. and should be determined by the end user. The recommended sample size is 0.4 mL. 6. Add Calcein AM to each sample and mix gently. For example, to stain a 0.4 mL sized sample with 10 μM Calcein AM, add 10 μL of the 400 μM stock solution. 7. Incubate approximately 1 hour. 8. Reconstitute 7-AAD with 260 μL DMSO, forming a 200X stock concentrate. 9. Add 7-AAD to each sample at 1:200 (e.g. add 2 μL to 400 μL of sample) . 10. Analzye with a flow cytometer or fluorescence microscope. Calcein AM excites at 494 nm and emits at 520 nm. 7-AAD excites at 546 nm and emits at 647 nm.

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