Products for Research Use Only
RegisterLogin

Magic Red Cathepsin B Assay Kit

CAT: 0013-GTR18821958Size: 25 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18821958Size:25 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Quantitatively monitor intracellular cathepsin B activity over time in vitro. The Magic Red substrate fluoresces red upon cleavage by active cathepsin enzymes. The signal can be analyzed using fluorescence microscopy or a fluorescence plate reader.
Sample Type
Cell culture
Storage Conditions
2-8°C
Notes
For research use only.
Applications Notes
1. Prepare samples and controls. 2. Reconstitute Magic Red by adding DMSO. 3. Dilute Magic Red 1:10 by adding diH2O. 4. Add 20 μL Magic Red to each sample (~ 500 μL aliquot of cultured cells) . 5. Incubate while protected from light. 6. Watch color start to develop within 15 minutes. 7. If desired, label with additional stains, such as Hoechst, DAPI, or an antibody. 8. If desired, fix cells. 9. Analyze with a fluorescence microscope or fluorescence plate reader. Magic Red has an optimal excitation at 592 nm and emission at 628 nm. If working with adherent cells, please see the manual for additional protocols.

Related Products

CatalogName
GTR18821953Magic Red Cathepsin L Assay Kit
GTR18821956Magic Red Cathepsin K Assay Kit
GTR18821955Magic Red Cathepsin K Assay Kit
GTR18821954Magic Red Cathepsin L Assay Kit
GTR18800599-01Magic RedTM Cathepsin B Assay Kit
GTR18800601-01Magic RedTM Cathepsin L Assay Kit
GTR18800600-01Magic RedTM Cathepsin K Assay Kit
GTR18821961Magic Red Caspase 3 & 7 Assay Kit
GTR18821960Magic Red Caspase 3 & 7 Assay Kit
GTR18800649-01Green Cathepsin B Assay Kit

Alternative Products

CatalogName
MBS258020-01Magic Red Cathepsin B Assay Kit
941Magic RedFluorescent Cathepsin L Assay Kit
MBS258031-01Magic Red Cathepsin L Assay Kit
940Magic RedFluorescent Cathepsin K Assay Kit
MBS258032-01Magic Red Cathepsin K Assay Kit
938Magic RedFluorescent Cathepsin B Assay Kit
942Magic Red® Fluorescent Cathepsin L Assay Kit
938Magic Red® Fluorescent Cathepsin B Assay Kit
940Magic Red® Fluorescent Cathepsin K Assay Kit
ICT-937Magic RedTM Cathepsin B Assay Kit (25 tests)

Popular Products