Products for Research Use Only

Human L-Carnitine ELISA Kit

CAT: 0013-GTR18821427-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0013-GTR18821427-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Human L-Carnitine ELISA Kit
Product Name Alternative
L-Carnitine
Reactivity
Human
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, urine, tissue homogenates
Sensitivity
0.119 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL