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Rabbit Thrombin/Antithrombin Complex (TAT) ELISA Kit

CAT: 0013-GTR18819949-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18819949-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thrombin/Antithrombin Complex (TAT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Thrombin/Antithrombin Complex (TAT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thrombin/Antithrombin Complex (TAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thrombin/Antithrombin Complex (TAT) in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Thrombin/Antithrombin Complex
Reactivity
Rabbit
Field of Research
Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Plasma
Sensitivity
0.121 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TAT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TAT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TAT in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL

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