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Pig Vimentin (VIM) ELISA Kit

CAT: 0013-GTR18815457-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18815457-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vimentin (VIM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Vimentin (VIM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vimentin (VIM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vimentin (VIM) in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Vimentin
UniProt
P02543
Reactivity
Porcine
Field of Research
Neuroscience; Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.61 ng/mL
References & Citations
María Botía et al. Potential new biomarkers in pig saliva for differentiating between the effects of different conditions of mixing and density at pre-slaughter: A preliminary proteomicstudy Anim Welf, (2026)
Pubmed id
41567522
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig VIM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig VIM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig VIM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig VIM in the samples is then determined by comparing the OD of the samples to the standard curve
Dynamic Range
1.57-100 ng/mL

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