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Mouse Eomesodermin (EOMES) ELISA Kit

CAT: 0013-GTR18814359-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18814359-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Eomesodermin (EOMES) .
Product Name Alternative
TBR2 ELISA Kit, T-Box Brain 2 ELISA Kit
UniProt
O54839
Reactivity
Mouse
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.111 ng/mL
Notes
For research use only.
Applications Notes
Standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse EOMES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse EOMES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse EOMES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse EOMES in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.32-20 ng/mL

UniProtKB · O54839

Eomesodermin homolog

EOMES_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O54839
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Eomes
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q3UPL1, Q52KJ1, Q8BN22, Q9JJL1, Q9QYG7
Protein keywords

Molecular function

ActivatorDevelopmental proteinDNA-binding

Biological process

Adaptive immunityDifferentiationGastrulationImmunityTranscriptionTranscription regulation

Coding sequence diversity

Alternative splicing

Cellular component

Nucleus

PTM

Phosphoprotein

Technical term

Reference proteome

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