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Mouse Tight Junction Protein 1 (TJP1) ELISA Kit

CAT: 0013-GTR18814351-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18814351-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tight Junction Protein 1 (TJP1) .
Product Name Alternative
ZO1 ELISA Kit, Zona Occludens 1 ELISA Kit, Zona occludens protein 1 ELISA Kit, Zonula occludens protein 1 ELISA Kit
UniProt
P39447
Reactivity
Mouse
Field of Research
Signal Transduction
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.053 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TJP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TJP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TJP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TJP1 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · P39447

Tight junction protein 1

ZO1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P39447
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tjp1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
E9QK00
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

Calmodulin-binding

Cellular component

Cell junctionCell membraneCytoplasmGap junctionMembraneTight junction

PTM

Phosphoprotein

Domain

RepeatSH3 domain

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