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Human Methotrexate (MTX) ELISA Kit

CAT: 0013-GTR18813992-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813992-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Human Methotrexate (MTX) ELISA Kit
Product Name Alternative
Methotrexate ELISA Kit, Amethopterin ELISA Kit
Reactivity
Human
Target
MTX
Field of Research
Pharmacology & Drug Discovery
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
4.57 ng/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MTX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTX in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
15.63-1000 ng/mL

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