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Mouse Adenosine (ADO) ELISA Kit

CAT: 0013-GTR18819921-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18819921-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adenosine (ADO) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Adenosine (ADO) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Adenosine (ADO), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Adenosine (ADO) in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Adenosine
UniProt
Q6PDY2
Reactivity
Mouse
Field of Research
Metabolism Research; Pharmacology & Drug Discovery
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Sensitivity
0.56 ng/mL
Notes
For research use only.
Applications Notes
Standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ADO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADO in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
1.57-100 ng/mL

UniProtKB · Q6PDY2

2-aminoethanethiol dioxygenase

AEDO_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q6PDY2
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ado
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
1.13.11.19
Processing
—
Secondary accessions
Q3U2E1
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

DioxygenaseOxidoreductase

Ligand

IronMetal-binding

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