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Human CD97 Antigen (CD97) ELISA Kit

CAT: 0013-GTR18814237-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18814237-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD97 (CD97 antigen) .
Product Name Alternative
CD97 ELISA Kit, CD97 antigen ELISA Kit, CD97 molecule ELISA Kit, Leukocyte antigen CD97 ELISA Kit, TM7LN1 ELISA Kit
UniProt
P48960
Reactivity
Human
Field of Research
Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.072 ng/mL
Notes
For research use only.
Applications Notes
Standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD97. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD97. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD97, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD97 in the samples is then determined by comparing the OD of the samples to the standard curve
Tested Applications
ELISA
Dynamic Range
0.16-10 ng/mL

UniProtKB · P48960

Adhesion G protein-coupled receptor E5

AGRE5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P48960
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
ADGRE5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8K7Z4, B2RBJ9, O00718, O76101, Q8NG72, Q8TBQ7
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

Calcium

Biological process

Cell adhesion

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

G protein-coupled receptorReceptorTransducer

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