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General Dihydrotestosterone (DHT) ELISA Kit

CAT: 0013-GTR18813528-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18813528-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
General Dihydrotestosterone (DHT) ELISA Kit
Product Name Alternative
5α-DHT ELISA Kit, 5α-Dihydrotestosterone ELISA Kit, Androstanolone ELISA Kit, Stanolone ELISA Kit
Reactivity
All
Target
DHT
Field of Research
Metabolism Research, Stem Cell & Developmental Biology
Assay Type
Competitive
Assay Performance Time
2h
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Sensitivity
13.7 pg/mL
Notes
For research use only.
Applications Notes
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHT in the samples is then determined by comparing the OD of the samples to the standard curve.
Tested Applications
ELISA
Dynamic Range
39.07-2500 pg/mL

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